p53 localizes to the nucleus following infection with RVFV. Dana Austin Alan Baer Lindsay Lundberg Nazly Shafagati Annalise Schoonmaker Aarthi Narayanan Taissia Popova Jean Jacques Panthier Fatah Kashanchi Charles Bailey Kylene Kehn-Hall 10.1371/journal.pone.0036327.g002 https://plos.figshare.com/articles/figure/_p53_localizes_to_the_nucleus_following_infection_with_RVFV_/313263 <p>A) HSAECs were either mock infected or infected with MP-12 (MOI 3.0). At 24 hours post infection the cells were washed in PBS without Ca and Mg, permeabilized with Triton X-100 and immunostained with anti-p53 primary antibody using Alexa-Fluor 568 secondary antibody and with anti-RVFV N protein antibody using Alexa-Fluor 488 secondary antibody. The nucleus was stained with DAPI. B) HSAECs were mock infected or infected with MP-12 (MOI 3.0) and collected at 24 hours post-infection for the creation of cytoplasmic and nuclear extracts, CE and NE. Western blot analysis was performed with antibodies against lamin, GAPDH, total p53 and RVFV N protein.</p> 2012-05-04 00:54:23 localizes nucleus