Soubeyrand, Sébastien Martinuk, Amy Lau, Paulina McPherson, Ruth Suppression of βTRCP increases <i>TRIB1</i> expression but does not affect its instability. <p>HeLaT1 cells incubated for 48 h with siRNA targeting CUL1, βTRCP or a non-target control were treated with vehicle (H<sub>2</sub>0) or CHX for 5 h. Parallel lysates were then analyzed by western blot and qPCR. A, qRT-PCR of RNA isolated from cells treated with the indicated siRNAs for 48 h. Quantification of corresponding transcript is shown, normalized to the non-target oligonucleotide (NT). B, western blot of extracts from silenced cells. Data shown are representative of 3 biological replicates. C, βTRCP and CUL1 suppression increase <i>TRIB1</i> transcript abundance. qRT-PCR of <i>TRIB1</i> in cells suppressed for the indicated siRNAs. In A and C, data represent the average of 2 biological replicates ± S.D.</p> TRIB 1 mRNA;Blocking proteasome function;TRIB 1 gene;TRIB 1 regulation;rescue protein instability;TRIB 1 expression;TRCP β suppression;TRIB 1 instability;CUL;TRIB 1 subcellular localization;CAD;TRIB 1 function;control TRIB 1;HEK 293T HeLa;TRIB 1 2016-03-28
    https://plos.figshare.com/articles/figure/Suppression_of_TRCP_increases_i_TRIB1_i_expression_but_does_not_affect_its_instability_/3132319
10.1371/journal.pone.0152346.g008