%0 Figure %A Zheng, Xiaojing %A Li, Yanqing %A Zhao, Junli %A Wang, Dongyang %A Xia, Haibin %A Mao, Qinwen %D 2016 %T Production and purification of recombinant hFAM76B-6His. %U https://plos.figshare.com/articles/figure/Production_and_purification_of_recombinant_hFAM76B_6His_/3132310 %R 10.1371/journal.pone.0152237.g001 %2 https://ndownloader.figshare.com/files/4872550 %K hFAM 76B %K homopolymeric histidine tracts %K FAM 76B murine %K 39 kDa protein %K Human Nuclear Protein FAM 76B Human FAM 76B %K FAM 76B HEK 293 cell line %K FAM 76B %K FAM 76B MAbs %K mouse FAM 76B %K laser confocal microscopy %K hFAM 76B protein %X

(A) His6-tagged hFAM76B protein was produced in E. coli BL21 (DE3), purified by Ni–NTA column and subjected to 10% SDS-PAGE. Coomassie blue staining revealed a band of 42 kDa, consistent with the predicted size of hFAM76B. Lane 1, protein molecular weight marker; lane 2, nonbound sample; lanes 3–5, samples eluted with 5 mM, 50 mM and 250 mM imidazole, respectively. (B) The purified protein (50 ng/lane) was confirmed by Western blot analysis with anti-6His antibody and rabbit polyclonal anti-hFAM76B antibody (Sigma, St. Louis, MO).

%I PLOS ONE