10.6084/m9.figshare.3119995.v1
Jenna D. Strickland
Jenna
D. Strickland
William R. Lefew
William
R. Lefew
James Crooks
James
Crooks
Diana Hall
Diana
Hall
Jayna NR Ortenzio
Jayna
NR Ortenzio
Kevin Dreher
Kevin
Dreher
Timothy J. Shafer
Timothy
J. Shafer
<i>In vitro</i> screening of metal oxide nanoparticles for effects on neural function using cortical networks on microelectrode arrays
Taylor & Francis Group
2016
69 nm CeO 2
TS
48 h
48 h recording
1 h exposure
25 nm TiO 2 NPs
MP
31 nm TiO 2
microelectrode arrays Nanoparticles
MEA
metal oxide nanoparticles
recording 1 h
metal oxide particles cause
GABAA antagonist bicuculline
BIC
AE
25 µ M
1288 nm CeO 2
2016-03-21 12:38:19
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/_i_In_vitro_i_screening_of_metal_oxide_nanoparticles_for_effects_on_neural_function_using_cortical_networks_on_microelectrode_arrays/3119995
<p>Nanoparticles (NPs) may translocate to the brain following inhalation or oral exposures, yet higher throughput methods to screen NPs for potential neurotoxicity are lacking. The present study examined effects of 5 CeO<sub>2</sub> (5– 1288 nm), and 4 TiO<sub>2</sub> (6–142 nm) NPs and microparticles (MP) on network function in primary cultures of rat cortex on 12 well microelectrode array (MEA) plates. Particles were without cytotoxicity at concentrations ≤50 µg/ml. After recording 1 h of baseline activity prior to particle (3–50 µg/ml) exposure, changes in the total number of spikes (TS) and # of active electrodes (#AEs) were assessed 1, 24, and 48 h later. Following the 48 h recording, the response to a challenge with the GABA<sub>A</sub> antagonist bicuculline (BIC; 25 µM) was assessed. In all, particles effects were subtle, but 69 nm CeO<sub>2</sub> and 25 nm TiO<sub>2</sub> NPs caused concentration-related decreases in TS following 1 h exposure. At 48 h, 5 and 69 nm CeO<sub>2</sub> and 25 and 31 nm TiO<sub>2</sub> decreased #AE, while the two MPs increased #AEs. Following BIC, only 31 nm TiO<sub>2</sub> produced concentration-related decreases in #AEs, while 1288 nm CeO<sub>2</sub> caused concentration-related increases in both TS and #AE. The results indicate that some metal oxide particles cause subtle concentration-related changes in spontaneous and/or GABA<sub>A</sub> receptor-mediated neuronal activity <i>in vitro</i> at times when cytotoxicity is absent, and that MEAs can be used to screen and prioritize nanoparticles for neurotoxicity hazard.</p>