TY - DATA T1 - Effect of Surface Functionalization of MCM-41-Type Mesoporous Silica Nanoparticles on the Endocytosis by Human Cancer Cells PY - 2006/11/22 AU - Igor Slowing AU - Brian G. Trewyn AU - Victor S.-Y. Lin UR - https://acs.figshare.com/articles/journal_contribution/Effect_of_Surface_Functionalization_of_MCM_41_Type_Mesoporous_Silica_Nanoparticles_on_the_Endocytosis_by_Human_Cancer_Cells/3046642 DO - 10.1021/ja0645943.s001 L4 - https://ndownloader.figshare.com/files/4752163 KW - Confocal fluorescence micrographs KW - GP KW - Human Cancer CellsWe KW - cancer cells KW - HeLa cells KW - AP KW - endosomal entrapment KW - uptake efficiency KW - immunocytochemistry study KW - Flow cytometry results KW - MCM KW - endocytosis mechanism KW - surface functionalities KW - Surface Functionalization KW - surface functionalization KW - FAP KW - GEGP KW - functionalized MSNs KW - 100 mM PBS buffer KW - intracellular delivery vehicles N2 - We have synthesized a series of MCM-41-type mesoporous silica nanoparticles (MSN). The surface of the MSNs are functionalized with 3-aminopropyl (AP), 3-guanidinopropyl (GP), 3-[N-(2-guanidinoethyl)guanidino]propyl (GEGP), and N-folate-3-aminopropyl (FAP). In contrast to the ζ-potential of −18.4 mV for FITC-MSN, the values of ζ-potential for AP-, GP-, GEGP-, and FAP-functionalized FITC-MSNs in 100 mM PBS buffer (pH 7.4) increased positively from −11.3, −10.6, −4.0, to +4.9 mV, respectively. The uptake efficiency, endocytosis mechanism, and biocompatibility of these organically functionalized MSNs were investigated with human cervical cancer cells (HeLa). Flow cytometry results suggested that the endocytosis of MSN could be manipulated by different surface functionalization. The immunocytochemistry study indicated that the uptake of these MSNs by HeLa cells was surface functional group dependent and involved several different mechanisms of endocytosis. Confocal fluorescence micrographs showed that the different surface functionalities of MSNs could also affect their ability to escape endosomal entrapment, which is a key factor in designing effective intracellular delivery vehicles. ER -