Zhu, Yichao Tian, Yinhui Du, Jun Hu, Zhenzhen Yang, Ling Liu, Jiaojing Gu, Luo Daam1 participates in the rearrangement of stress fibers in MDA-MB-231 and MCF-7 cells. <p>(A) <i>N</i>-Daam1 and C3 transferase disrupted the formation of actin stress fibers in MDA-MB-231 cells. MDA-MB-231 cells were transiently transfected with <i>N</i>-Daam1 or pre-treated with 10 ng/µL C3 transferase for 1 h. MDA-MB-231 cells as well as transiently transfected Daam1 mutants were grown on cover slips, and then incubated in culture medium containing 500 ng/mL rWnt5a for 4 h. Subsequently, cells were fixed and F-actin organization was analyzed by phalloidin staining. (C) ΔDAD-Daam1 enhanced the formation/maintenance of actin stress fibers in MCF-7. MCF-7 cells were transiently transfected with ΔDAD-Daam1, and then incubated in culture medium in the absence of Wnt5a. Magnification, ×400. (B, D) The percentage of formation of actin stress fibers was determined in MDA-MB-231 cells (B) and MCF-7 cells (D), as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037823#s2" target="_blank">Materials and Methods</a>. The average is shown as mean ± s.d.</p> participates;rearrangement;fibers;mda-mb-231;mcf-7 2012-05-24
    https://plos.figshare.com/articles/figure/_Daam1_participates_in_the_rearrangement_of_stress_fibers_in_MDA_MB_231_and_MCF_7_cells_/304072
10.1371/journal.pone.0037823.g006