Free energy diagram for the conformational spread model a) symmectric case: the free energy of conformational change is <i>±E<sub>A</sub></i>. MaQi V. Nicolau JrDan MainiPhilip K. BerryRichard M. BaiFan 2013 <p>The free energy of CheY-P binding is dependent on the CheY-P concentration as shown, with <i>E<sub>C</sub> = −ln(c/c<sub>0.5</sub>)</i>. If <i>E<sub>C</sub>>0</i>, the inactive state becomes more highly populated and hence CW bias <0.5, while <i>E<sub>C</sub><0</i> similarly implies CW bias >0.5. b) asymmetric case: the free energy of conformational change is <i>±E<sub>A</sub><sup>0</sup></i> when a protomer is unliganded and <i>±E<sub>A</sub><sup>1</sup></i> when it is liganded. c) coupling energy <i>E<sub>J</sub></i> between neighboring protomers.</p>