GTPs and SFN altered epigenetic enzymes expression and their activity in ERα-negative breast cancer cells. Syed M. Meeran Shweta N. Patel Yuanyuan Li Samriddhi Shukla Trygve O. Tollefsbol 10.1371/journal.pone.0037748.g003 https://plos.figshare.com/articles/figure/_GTPs_and_SFN_altered_epigenetic_enzymes_expression_and_their_activity_in_ER_945_negative_breast_cancer_cells_/303301 <p>Breast cancer MDA-MB-231 cells were treated with the indicated concentration of GTPs and SFN alone or in combinations of both for 3 days. Nuclear extracts were prepared and 20 µg of protein was used to estimate DNMTs (<i>panel A</i>), HDAC and HATs (<i>panel B</i>) activities using the colorimetric assay kit as described under <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037748#s2" target="_blank"><i>Materials and Methods</i></a>. Non-treated MCF-7 cells were used as a positive control. Values are representative of three independent experiments and represented as percent control ± SD; statistical significance against nontreated MDA-MB-231 control, <sup>*</sup>P<0.05. <i>C)</i> Effect of GTPs and SFN alone or in combinations of both on DNMTs, SUV39H1 and HDACs expression in ERα-negative human breast cancer MDA-MB-231 cells. Cell lysates were prepared at 3 days of post-treatments at the indicated doses followed by western blotting to analyze DNMTs (DNMT1, DNMT3a and DNMT3b), HDACs (HDAC1, HDAC4 and HDAC6) and SUV39H1 expression. Non-treated MCF-7 cells were used as a positive control. Actin was used as an equal loading control. <i>D)</i> Graphical representations are indicative of relative band intensity of DNMTs expression in MDA-MB-231 cells, normalized with β-actin. Values are mean band intensity of three independent blot ± SD.</p> 2013-02-20 04:41:35 sfn altered epigenetic enzymes cancer