10.1021/ja0660958.s001
Shuzo Matsumoto
Shuzo
Matsumoto
Akira Yane
Akira
Yane
Satoru Nakashima
Satoru
Nakashima
Masaki Hashida
Masaki
Hashida
Masayuki Fujita
Masayuki
Fujita
Yuji Goto
Yuji
Goto
Satoshi Takahashi
Satoshi
Takahashi
A Rapid Flow Mixer with 11-μs Mixing Time Microfabricated by a
Pulsed-Laser Ablation Technique: Observation of a Barrier-Limited Collapse
in Cytochrome <i>c</i> Folding
American Chemical Society
2007
Cytochrome c FoldingProteins
dynamic
Mixing Time Microfabricated
Rapid Flow Mixer
collapse
femtosecond laser ablation technique
cytochrome c
mixer
2007-04-04 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/A_Rapid_Flow_Mixer_with_11_s_Mixing_Time_Microfabricated_by_a_Pulsed_Laser_Ablation_Technique_Observation_of_a_Barrier_Limited_Collapse_in_Cytochrome_i_c_i_Folding/3014428
Proteins with more than ∼100 residues usually demonstrate a rapid and significant collapse as the earliest event in their folding dynamics; however, the limit in the time resolution of available experimental methods precluded us from conducting a detailed analysis of the event. To investigate the collapse in a real time, a new rapid flow mixer was developed by using a femtosecond laser ablation technique, which achieved a mixing time of 11 μs. By using the developed mixer, the collapse dynamics of cytochrome <i>c</i>, initiated by pH jumps after the solution mixing, was investigated. The time domain within 100 μs, which was previously unobservable, was clearly resolved and demonstrated that the process can be approximated by a single-exponential process. The results support the presence of a single kinetic barrier in the initial collapse of cytochrome <i>c</i>.