Transcription and translation in mouse blastocysts.
Telma C. Esteves
Olympia E. Psathaki
Martin J. Pfeiffer
Sebastian T. Balbach
Dagmar Zeuschner
Hiroshi Shitara
Hiromichi Yonekawa
Marcin Siatkowski
Georg Fuellen
Michele Boiani
10.1371/journal.pone.0036850.g005
https://plos.figshare.com/articles/figure/_Transcription_and_translation_in_mouse_blastocysts_/299163
<p>mRNA levels were determined for pools of six ICSI and SCNT blastocysts (in duplicate), by quantitative RT-PCR, over two independent experiments. Technical replicates were determined three times for each sample, and results were normalized to Flag-Oct4 mRNA. (A) dCT values for transcripts in mtGFP-Tg SCNT, compared to ICSI. (B) dCT values for Gapdh and Actb mRNA, as well as protein levels (by immunocytochemistry), in wild-type SCNT relative to ICSI. Gapdh and Actb levels were significantly different, both for mRNA (t-test, <i>p</i> = 0,001 and <i>p</i> = 1,29E-08, respectively) and protein (t-test, <i>p</i> = 0.006 and <i>p</i> = 0.04, respectively). Box plot: fluorescence intensities distribution in the blastocyst; top and bottom lines: inter-quartile range; middle line: median; whiskers: range of variation limited to 1.5 times inter-quartile range.</p>
2013-02-20 04:19:22
genetics and genomics
molecular biology
developmental biology
Biochemistry