%0 Figure %A Aggarwal, Anupriya %A L. Iemma, Tina %A Shih, Ivy %A P. Newsome, Timothy %A McAllery, Samantha %A L. Cunningham, Anthony %A G. Turville, Stuart %D 2012 %T High VF frequencies correlate with the efficiency of immature DC viral transfer. %U https://plos.figshare.com/articles/figure/_High_VF_frequencies_correlate_with_the_efficiency_of_immature_DC_viral_transfer_/299146 %R 10.1371/journal.ppat.1002762.g007 %2 https://ndownloader.figshare.com/files/628664 %K vf %K frequencies %K correlate %K immature %K dc %K viral %X

(A) Schematic of the DC-CD4 T cell transfer assay. DC are infected with either HIVWT (high VF frequency) or HIV-NEF-ve (low VF frequency) pseudotyped with the VSVg glycoprotein, to ensure equal infection frequencies. After 4 days, DC infections are normalized to 5% with uninfected DC. Normalized populations are serially diluted below 1 infected DC per co-culture. 4 days post co-culture, CD4 T cell infections are resolved by staining cells for of HIV capsid and resolution by flow cytometry. (B) Flow cytometry detection of HIV p24 within CD4 T cell recipients when input infected DC are limiting (upper panel) versus (C) when input infected CD4 T cells are limiting (lower panel). Approximate infected cell number input into co-cultures is indicated at “Approx. Input*” on the X-axis. CD4 T cell infection frequencies are detected by the accumulation of a high HIV p24 population as indicated by the square gate in each dot-plot. Statistical difference is presented in upper HIV WT panels, and is calculated from data acquired from the same assay performed in triplicate. CD4 T cell infection frequency versus infected donor input is further summarized in right panels for two representative donors. Standard deviations represent co-cultures in triplicate. Data is representative of n = 8 independent donors.

%I PLOS Pathogens