10.1021/jp073147m.s002
Hui Tong
Hui
Tong
Yuning Hong
Yuning
Hong
Yongqiang Dong
Yongqiang
Dong
Matthias Häussler
Matthias
Häussler
Zhen Li
Zhen
Li
Jacky W. Y. Lam
Jacky
W. Y. Lam
Yuping Dong
Yuping
Dong
Herman H.-Y. Sung
Herman H.-Y.
Sung
Ian D. Williams
Ian D.
Williams
Ben Zhong Tang
Ben Zhong
Tang
Protein Detection and Quantitation by Tetraphenylethene-Based Fluorescent Probes with
Aggregation-Induced Emission Characteristics
American Chemical Society
2007
protein detection
salt 3
fluorescence properties
AIE effect
solution state
BSA
Protein Detection
bi
TPE molecules
intramolecular rotations
model protein
novel process
dye molecules
serum albumin
2007-10-11 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Protein_Detection_and_Quantitation_by_Tetraphenylethene_Based_Fluorescent_Probes_with_Aggregation_Induced_Emission_Characteristics/2981296
Three functionalized derivatives of tetraphenylethylene (TPE), namely, 1,2-bis(4-methoxyphenyl)-1,2-diphenylethene (<b>1</b>), 1,2-bis(4-hydroxyphenyl)-1,2-diphenylethene (<b>2</b>), and 1,2-bis[4-(3-sulfonatopropoxyl)phenyl]-1,2-diphenylethene sodium salt (<b>3</b>), were synthesized and their fluorescence properties were
investigated. All the TPE molecules are nonluminescent in the solution state but are induced to emit efficiently
by aggregate formation. This novel process of aggregation-induced emission (AIE) is rationalized to be caused
by the restriction of intramolecular rotations of the dye molecules in the aggregate state. The possibility of
utilizing the AIE effect for protein detection and quantification is explored using bovine serum albumin (BSA)
as a model protein, with salt <b>3</b> being found to perform as a stable, sensitive, and selective bioprobe.