Wong, Joyce Y. Kuhl, Tonya L. Dynamics of Membrane Adhesion:  The Role of Polyethylene Glycol Spacers, Ligand−Receptor Bond Strength, and Rupture Pathway<sup>†</sup> Biological adhesion typically occurs through discrete cross bridges between complementary molecules on adjacent membranes. Here we report quantitative measurements of the binding distance between a lipid membrane functionalized with ligands on flexible polymer tether chains and a second membrane bearing complementary receptors using the surface force apparatus technique. The binding distance is shown to increase as a function of polymer tether length. Upon separation, adhesive failure occurs not at the strong ligand−receptor bond but primarily through the mechanical pullout of cross-bridging polymer tethers from the membrane. We summarize these measurements of complementary membrane adhesion dynamics using an energy-state diagram that encompasses the energetics of the polymer tether, ligand−receptor bond strength, and number of cross bridges formed. binding distance;measurement;ligand;lipid membrane functionalized;polymer tether chains;surface force apparatus technique;membrane adhesion dynamics;polymer tether length;Polyethylene Glycol Spacers;bond 2008-02-19
    https://acs.figshare.com/articles/journal_contribution/Dynamics_of_Membrane_Adhesion_The_Role_of_Polyethylene_Glycol_Spacers_Ligand_Receptor_Bond_Strength_and_Rupture_Pathway_sup_sup_/2956552
10.1021/la702357a.s002