10.1021/jp7107158.s002
Randy A. Burgett
Randy A.
Burgett
Xiaofeng Bao
Xiaofeng
Bao
Frederick A. Villamena
Frederick A.
Villamena
Superoxide Radical Anion Adduct of 5,5-Dimethyl-1-pyrroline <i>N</i>-Oxide (DMPO). 3. Effect
of Mildly Acidic pH on the Thermodynamics and Kinetics of Adduct Formation
American Chemical Society
2008
DMPO
pKa
PCM
Mildly Acidic pH
Adduct FormationThe nitrone
DFT
1 H
13 C NMR
Superoxide Radical Anion Adduct
2008-03-20 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Superoxide_Radical_Anion_Adduct_of_5_5_Dimethyl_1_pyrroline_i_N_i_Oxide_DMPO_3_Effect_of_Mildly_Acidic_pH_on_the_Thermodynamics_and_Kinetics_of_Adduct_Formation/2950123
The nitrone, 5,5-dimethylpyrroline <i>N</i>-oxide (DMPO), is a commonly used spin trap for the detection of
superoxide radical anion (O<sub>2</sub><sup>•-</sup>) using electron paramagnetic resonance spectroscopy. This work investigates
the reactivity of DMPO to O<sub>2</sub><sup>•-</sup> in mildly acidic pH (5.0−7.0). Mild acidity is characteristic of acidosis and
has been observed in hypoxic systems, e.g., ischemic organs and cancer cells. Although the established p<i>K</i><sub>a</sub>
for O<sub>2</sub><sup>•-</sup> is 4.8, the p<i>K</i><sub>a</sub> for DMPO is unknown. The p<i>K</i><sub>a</sub> of the conjugate acid of DMPO was determined to
be 6.0 using potentiometric, spectrophotometric, <sup>1</sup>H and <sup>13</sup>C NMR, and computational methods. <sup>1</sup>H and <sup>13</sup>C
NMR were employed to investigate the site of protonation. An alternative mechanism for the spin trapping
of O<sub>2</sub><sup>•-</sup> in mildly acidic pH was proposed, which involves protonation of the oxygen to form the <i>N</i>-hydroxy
imino cation and subsequent addition of O<sub>2</sub><sup>•-</sup>. The exoergicity of O<sub>2</sub><sup>•-</sup> addition to protonated DMPO was
rationalized using density functional theory (DFT) at the PCM/B3LYP/6-31+G**//B3LYP/6-31G* level of
theory.