10.1021/ac800556f.s001
Liu Yang
Liu
Yang
Huimin Li
Huimin
Li
Kemin Wang
Kemin
Wang
Weihong Tan
Weihong
Tan
Wenjuan Yang
Wenjuan
Yang
Jing Zheng
Jing
Zheng
Atomic Force Microscopy Study of the Effect of Pulsed Electric Field on <i>Staphylococcus epidermidis</i>
American Chemical Society
2008
image
PEF
Atomic Force Microscopy Study
AFM
force measurement
envelope
Pulsed Electric Field
Staphylococcus epidermidisThe effect
chemical composition change
2008-08-15 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Atomic_Force_Microscopy_Study_of_the_Effect_of_Pulsed_Electric_Field_on_i_Staphylococcus_epidermidis_i_/2920402
The effect of a pulsed electric field (PEF) on <i>Staphylococcus epidermidis</i> was investigated by using an atomic force microscopy (AFM) image and force measurement under liquid. The changes in the bacterial envelope were probed in situ before and after applying different dosages of PEF. Our results indicated that PEF induced the changes of bacterial peptidoglycan layer and the exposure of plasma membrane. This conclusion was further confirmed by two control experiments: the effect of the lysozyme or heat on the bacterial envelope. Furthermore, our results demonstrated that AFM analysis including image and force measurement is helpful to explain the relationship between the chemical composition change of the cellular envelope and the external stimulation.