10.1021/jacs.5b11602.s002
Yuan Chang
Yuan
Chang
Zheng Liu
Zheng
Liu
Yun Zhang
Yun
Zhang
Kornelia Galior
Kornelia
Galior
Jeffery Yang
Jeffery
Yang
Khalid Salaita
Khalid
Salaita
A General Approach for Generating Fluorescent Probes
to Visualize Piconewton Forces at the Cell Surface
American Chemical Society
2016
phase peptide synthesis
receptor
SPPS
Cell SurfaceMechanical forces
ECM
tension probes
mapping
approach
NCL
2016-02-12 00:00:00
Media
https://acs.figshare.com/articles/media/A_General_Approach_for_Generating_Fluorescent_Probes_to_Visualize_Piconewton_Forces_at_the_Cell_Surface/2874850
Mechanical
forces between cells and their extracellular matrix
(ECM) are mediated by dozens of different receptors. These biophysical
interactions play fundamental roles in processes ranging from cellular
development to tumor progression. However, mapping the spatial and
temporal dynamics of tension among various receptor–ligand
pairs remains a significant challenge. To address this issue, we have
developed a synthetic strategy to generate modular tension probes
combining the native chemical ligation (NCL) reaction with solid phase
peptide synthesis (SPPS). In principle, this approach accommodates
virtually any peptide or expressed protein amenable to NCL. We generated
a small library of tension probes displaying different ligands, flexible
linkers, and fluorescent reporters, enabling the mapping of integrin
and cadherin tension, and demonstrating the first example of long-term
(∼3 days) molecular tension imaging. This approach provides
a toolset to better understand mechanotransduction events fundamental
to cell biology.