Papoti, Vassiliki T. Tsimidou, Maria Z. Impact of Sampling Parameters on the Radical Scavenging Potential of Olive (Olea europaea L.) Leaves The impact of sampling parameters, that is, cultivar, leaf age, and sampling date, on the radical scavenging potential of olive leaf extracts was examined via the DPPH<sup>•</sup> and other assays. Total phenol content was estimated colorimetrically and by fluorometry, whereas phenol composition was assessed by RP-HPLC coupled with diode array, fluorometric, and MS detection systems. Oleuropein was not always the major leaf constituent. Considerable differences noted in individual phenol levels (hydroxytyrosol, oleuropein and other secoiridoids, verbascoside, and flavonoids) among samples were not reflected either in the total phenol content or in the radical scavenging potential of the extracts. It can be suggested that olive leaf is a robust source of radical scavengers throughout the year and that differentiation in the levels of individual components depends rather on sampling period than on cultivar or age. The latter does not present predictable regularity. Exploitation of all types of leaves expected in an olive tree shoot for the extraction of bioactive compounds is feasible. DPPH;leaf constituent;phenol content;Considerable differences;Total phenol content;impact;leaf age;diode array;leaf extracts;MS detection systems;phenol composition;sampling parameters;sampling period;phenol levels;bioactive compounds;cultivar;sampling date;Sampling Parameters;Radical Scavenging 2009-05-13
    https://acs.figshare.com/articles/journal_contribution/Impact_of_Sampling_Parameters_on_the_Radical_Scavenging_Potential_of_Olive_Olea_europaea_L_Leaves/2857732
10.1021/jf900171d.s001