Hyaluronic Acid−Quantum Dot Conjugates for <i>In Vivo</i> Lymphatic Vessel Imaging
Suk Ho Bhang
Nayoun Won
Tae-Jin Lee
Ho Jin
Jutaek Nam
Joonhyuck Park
Hyokyun Chung
Hyun-Seo Park
Yung-Eun Sung
Sei Kwang Hahn
Byung-Soo Kim
Sungjee Kim
10.1021/nn900138d.s001
https://acs.figshare.com/articles/media/Hyaluronic_Acid_Quantum_Dot_Conjugates_for_i_In_Vivo_i_Lymphatic_Vessel_Imaging/2848417
A simple and novel electrostatic coupling method is reported, which provides a hyaluronic acid−quantum dot conjugate (HA−QD) that is colloidally stable and size-tunable from 50 to 120 nm. The HA−QDs show cancer targeting efficiency, which suggests diagnostic and imaging applications. The conjugates are also demonstrated for the fluorescence staining capability for lymphatic vessels <i>in vitro</i> and <i>in vivo</i>. Using the HA−QDs in a small animal model, lymphatic vessels are visualized real-time <i>in vivo</i> for days. Comprehensive cytotoxicity evaluations are made for the conjugates and the unconjugated counterpart. The HA−QDs showcase the potentials toward cancer imaging and real-time visualization of changes in lymphatic vessels such as lymphangiogenesis.
2009-06-23 00:00:00
fluorescence staining capability
vivo
conjugate
HA
Vivo Lymphatic Vessel ImagingA
Comprehensive cytotoxicity evaluations
lymphatic vessels
imaging