10.1021/ac901008c.s001
Virginia Vandelinder
Virginia
Vandelinder
Allan Chris M. Ferreon
Allan
Chris M. Ferreon
Yann Gambin
Yann
Gambin
Ashok A. Deniz
Ashok A.
Deniz
Alex Groisman
Alex
Groisman
High-Resolution Temperature−Concentration Diagram of α-Synuclein Conformation Obtained from a Single Förster Resonance Energy Transfer Image in a Microfluidic Device
American Chemical Society
2009
medium conditions
diagram
synuclein
microfluidic device
protein conformations
interrogation area
Fo
FRET
SDS
2009-08-15 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/High_Resolution_Temperature_Concentration_Diagram_of_Synuclein_Conformation_Obtained_from_a_Single_Fo_rster_Resonance_Energy_Transfer_Image_in_a_Microfluidic_Device/2835223
We present a microfluidic device for rapid and efficient determination of protein conformations in a range of medium conditions and temperatures. The device generates orthogonal gradients of concentration and temperature in an interrogation area that fits into the field of view of an objective lens with a numerical aperture of 0.45. A single Förster resonance energy transfer (FRET) image of the interrogation area containing a dual-labeled protein provides a 100 × 100 point map of the FRET efficiency that corresponds to a diagram of protein conformations in the coordinates of temperature and medium conditions. The device is used to explore the conformations of α-synuclein, an intrinsically disordered protein linked to Parkinson’s and Alzheimer’s diseases, in the presence of a binding partner, the lipid-mimetic sodium dodecyl sulfate (SDS). The experiment provides a diagram of conformations of α-synuclein with 10 000 individual data points in a range of 21−47 °C and 0−2.5 mM SDS. The diagram is consistent with previous reports but also reveals new conformational transitions that would be difficult to detect with conventional techniques. The microfluidic device can potentially be used to study other biomolecular and soft-matter systems.