10.1021/ac902978u.s001
Pavel A. Levashov
Pavel A.
Levashov
Sergey A. Sedov
Sergey A.
Sedov
Stepan Shipovskov
Stepan
Shipovskov
Natalia G. Belogurova
Natalia G.
Belogurova
Andrey V. Levashov
Andrey V.
Levashov
Quantitative Turbidimetric Assay of Enzymatic Gram-Negative Bacteria Lysis
American Chemical Society
2010
analysis
coli
pH
bacteria
LysisIn
8.9
0.03 M NaCl
enzyme
mechanism
evaluation
method
assay
Enzymatic
Technical
determination
Quantitative Turbidimetric Assay
lysi
lysozyme
bacteriolytic activity
turbidimetric
Bacteria
reactivity
2010-03-01 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Quantitative_Turbidimetric_Assay_of_Enzymatic_Gram_Negative_Bacteria_Lysis/2788447
In this Technical Note, the quantitative turbidimetric assay for determination of the bacteriolytic activity of enzymes with gram-negative bacteria is proposed. The reactivity of hen white-egg lysozyme toward gram-negative <i>E. coli</i> intact cells was studied. It was found that the highest lysis rate occurred at pH 8.9 in the system containing 0.03 M NaCl. The mechanism of the reaction is discussed and applied for the quantitative evaluation of the reaction rate. The proposed method enables fast, reliable, and reproducible analysis of bacteriolytic activity of lysozyme with gram-negative bacteria.