10.1021/ac902978u.s001 Pavel A. Levashov Pavel A. Levashov Sergey A. Sedov Sergey A. Sedov Stepan Shipovskov Stepan Shipovskov Natalia G. Belogurova Natalia G. Belogurova Andrey V. Levashov Andrey V. Levashov Quantitative Turbidimetric Assay of Enzymatic Gram-Negative Bacteria Lysis American Chemical Society 2010 analysis coli pH bacteria LysisIn 8.9 0.03 M NaCl enzyme mechanism evaluation method assay Enzymatic Technical determination Quantitative Turbidimetric Assay lysi lysozyme bacteriolytic activity turbidimetric Bacteria reactivity 2010-03-01 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Quantitative_Turbidimetric_Assay_of_Enzymatic_Gram_Negative_Bacteria_Lysis/2788447 In this Technical Note, the quantitative turbidimetric assay for determination of the bacteriolytic activity of enzymes with gram-negative bacteria is proposed. The reactivity of hen white-egg lysozyme toward gram-negative <i>E. coli</i> intact cells was studied. It was found that the highest lysis rate occurred at pH 8.9 in the system containing 0.03 M NaCl. The mechanism of the reaction is discussed and applied for the quantitative evaluation of the reaction rate. The proposed method enables fast, reliable, and reproducible analysis of bacteriolytic activity of lysozyme with gram-negative bacteria.