TY - DATA T1 - Space filling properties of the developing bronchial tree. PY - 2012/07/23 AU - Pierre Blanc AU - Karen Coste AU - Pierre Pouchin AU - Jean-Marc Azaïs AU - Loïc Blanchon AU - Denis Gallot AU - Vincent Sapin UR - https://plos.figshare.com/articles/figure/_Space_filling_properties_of_the_developing_bronchial_tree_/277607 DO - 10.1371/journal.pone.0041643.g007 L4 - https://ndownloader.figshare.com/files/6709128 KW - lung tissues mechanics KW - Mouse Lung Branching Morphogenesis Mammalian airways KW - E 13.5 mouse KW - lung epithelial tree KW - lung lobe morphogenesis KW - ramified tree-like structures KW - right cranial lobe structure KW - mesenchyme KW - vivo morphometric analysis KW - variation KW - epithelial tree N2 - (A) Spheres (blue) are allowed to grow at the bud tips until they reach one another and/or the lobe surface. Given these conditions, spheres show only slight differences of diameters. The minimal sphere radius within a lobe is used as in vivo space filling criterion (Kiv). Arrows indicate intricate spheres at site undergoing bifurcation. The gaps between the spheres are predictive of the next branching sites: dorsal side-branches (doted circles) and posterior side-branch (star). (B) Kiv is given as percentage of the optimal value approximated by the best random value BRV (see Material and Methods). Although the best random spacing is geometrically overrated (see Material and Methods), all the Kiv values are higher than 80% of BRV at E11 (red), E12 (purple) and E13 (green). (C) From E11 to E13, Kiv (blue bars) are comprised between the 99th BRV percentile (light grey bars) and the BRV value (dark grey bars), except for one E13 lobe, demonstrating that new bud tips are not distributed at random and tend to fill homogeneously the available space in the mesenchyme. Scale bar 100 µm. ER -