Surface-Initiated Assembly of Protein Nanofabrics
Adam W. Feinberg
Kevin Kit Parker
10.1021/nl100998p.s001
https://acs.figshare.com/articles/journal_contribution/Surface_Initiated_Assembly_of_Protein_Nanofabrics/2762734
Cells and tissues are self-organized within an extracellular matrix (ECM) composed of multifunctional, nano- to micrometer scale protein fibrils. We have developed a cell-free, surface-initiated assembly technique to rebuild this ECM structure in vitro. The matrix proteins fibronectin, laminin, fibrinogen, collagen type I, and collagen type IV are micropatterned onto thermosensitive surfaces as 1 to 10 nm thick, micrometer to centimeter wide networks, and released as flexible, free-standing nanofabrics. Independent control of microstructure and protein composition enables us to engineer the mechanical and chemical anisotropy. Fibronectin nanofabrics are highly extensible (>4-fold) and serve as scaffolds for engineering synchronously contracting, cardiac muscle; demonstrating biofunctionality comparable to cell-generated ECM.
2010-06-09 00:00:00
thermosensitive surfaces
ECM structure
extracellular matrix
Protein NanofabricsCells
engineering synchronously contracting
matrix proteins fibronectin
Independent control
micrometer scale protein fibrils
protein composition
collagen type
collagen type IV
Fibronectin nanofabrics
chemical anisotropy
10 nm