Itoh, Tomohiro Tsukane, Mariko Koike, Minako Nakamura, Chizu Ohguchi, Kenji Ito, Masafumi Akao, Yukihiro Koshimizu, Seiichi Nozawa, Yoshinori Wakimoto, Toshiyuki Nukaya, Haruo Suwa, Yoshihide Inhibitory Effects of Whisky Congeners on IgE-Mediated Degranulation in Rat Basophilic Leukemia RBL-2H3 Cells and Passive Cutaneous Anaphylaxis Reaction in Mice Whisky is matured in oak casks. Many nonvolatile substances (whisky congeners, WC) seep from the oak cask during the maturing process. In this study, three antiallergic agents (syringaldehyde, SA; lyoniresinol, Lyo; and ellagic acid, EA) were isolated from WC. Treatment with SA, Lyo, and EA reduced the elevation of intracellular free Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]i) and intracellular ROS production caused by FcεRI activation. The inhibitions of the elevation of [Ca<sup>2+</sup>]i and intracellular ROS production by SA and Lyo were mainly due to the suppression of the NADPH oxidase activity and scavenging of the produced radical, respectively. On the other hand, EA inactivated spleen tyrosine kinase and led to the inhibition of the elevation of [Ca<sup>2+</sup>]i and intracellular ROS production. Furthermore, it was found that WC strongly inhibited IgE binding to the FcεRIα chain, whereas SA, Lyo, and EA did not indicate this inhibitory effect. These results suggest that WC inhibits allergic reactions through multiple mechanisms. To disclose the in vivo effects of WC, SA, Lyo, and EA, these compounds were administered to type I allergic model mice, and the passive cutaneous anaphylaxis (PCA) reaction was measured. These compounds remarkably suppressed the PCA reaction. Taken together, these findings suggest that WC seemed to be beneficial to ameliorate allergic reactions. WC;antiallergic agents;ellagic acid;Fc εRI chain;Whisky Congeners;elevation;model mice;NADPH oxidase activity;IgE binding;cutaneous anaphylaxis;Lyo;PCA reaction;vivo effects;EA;spleen tyrosine kinase;oak casks;Fc εRI activation;SA;Many nonvolatile substances;oak cask;Inhibitory Effects;whisky congeners;Passive Cutaneous Anaphylaxis Reaction;RBL;intracellular ROS production 2010-06-23
    https://acs.figshare.com/articles/journal_contribution/Inhibitory_Effects_of_Whisky_Congeners_on_IgE_Mediated_Degranulation_in_Rat_Basophilic_Leukemia_RBL_2H3_Cells_and_Passive_Cutaneous_Anaphylaxis_Reaction_in_Mice/2758543
10.1021/jf100998c.s001