10.1021/ac101336d.s001 Yong Luo Yong Luo Wei Sun Wei Sun Yan Gu Yan Gu Gufeng Wang Gufeng Wang Ning Fang Ning Fang Wavelength-Dependent Differential Interference Contrast Microscopy: Multiplexing Detection Using Nonfluorescent Nanoparticles American Chemical Society 2010 multiplexing detection Interference Contrast Microscopy nanoparticle nanoprobe Nonfluorescent NanoparticlesThe wavelength dependence Multiplexing DIC contrast spectrum DIC microscopy kind 2010-08-01 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Wavelength_Dependent_Differential_Interference_Contrast_Microscopy_Multiplexing_Detection_Using_Nonfluorescent_Nanoparticles/2747464 The wavelength dependence of plasmonic nanoparticles’ contrasts in differential interference contrast (DIC) microscopy has been exploited previously for unambiguous identification and dynamic tracking of these nanoprobes in complex environments (<i>Anal. Chem.</i> <b>2009</b>, <i>81</i>, 9203−9208). In the present study, the suitability of multiplexing detection in DIC microscopy was investigated systematically with 19 kinds of nanoparticles of different materials and/or sizes. A unique DIC contrast spectrum was found for each kind of nanoparticle. Multiplexing detection was accomplished by measuring DIC contrasts at a minimum of two specific illumination wavelengths. The main advantages of DIC microscopy for multiplexing detection over other nonfluorescence techniques, such as dark field microscopy and surface-enhanced Raman scattering, were demonstrated by differentiating four kinds of nanoparticles on the cell membrane while providing high-contrast images of both the nanoprobes and cell features.