10.1021/ac101336d.s001
Yong Luo
Yong
Luo
Wei Sun
Wei
Sun
Yan Gu
Yan
Gu
Gufeng Wang
Gufeng
Wang
Ning Fang
Ning
Fang
Wavelength-Dependent Differential Interference Contrast Microscopy: Multiplexing Detection Using Nonfluorescent Nanoparticles
American Chemical Society
2010
multiplexing detection
Interference Contrast Microscopy
nanoparticle
nanoprobe
Nonfluorescent NanoparticlesThe wavelength dependence
Multiplexing
DIC contrast spectrum
DIC microscopy
kind
2010-08-01 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Wavelength_Dependent_Differential_Interference_Contrast_Microscopy_Multiplexing_Detection_Using_Nonfluorescent_Nanoparticles/2747464
The wavelength dependence of plasmonic nanoparticles’ contrasts in differential interference contrast (DIC) microscopy has been exploited previously for unambiguous identification and dynamic tracking of these nanoprobes in complex environments (<i>Anal. Chem.</i> <b>2009</b>, <i>81</i>, 9203−9208). In the present study, the suitability of multiplexing detection in DIC microscopy was investigated systematically with 19 kinds of nanoparticles of different materials and/or sizes. A unique DIC contrast spectrum was found for each kind of nanoparticle. Multiplexing detection was accomplished by measuring DIC contrasts at a minimum of two specific illumination wavelengths. The main advantages of DIC microscopy for multiplexing detection over other nonfluorescence techniques, such as dark field microscopy and surface-enhanced Raman scattering, were demonstrated by differentiating four kinds of nanoparticles on the cell membrane while providing high-contrast images of both the nanoprobes and cell features.