Jujuboside B increased intracellular Ca<sup>2+</sup> by inducing extracellular Ca<sup>2+</sup> to inflow through TRPC channels. Yixiu Zhao Xin Zhang Jiannan Li Yu Bian Miaomiao Sheng Bin Liu Zidong Fu Yan Zhang Baofeng Yang 10.1371/journal.pone.0149386.g007 https://plos.figshare.com/articles/figure/Jujuboside_B_increased_intracellular_Ca_sup_2_sup_by_inducing_extracellular_Ca_sup_2_sup_to_inflow_through_TRPC_channels_/2733658 <p>(A) Representative confocal image showed that Jujuboside B induced a rapid intracellular Ca<sup>2+</sup> elevation in HAECs instantaneously. (B) Fluorescence intensity curves showed that Jujuboside B increased intracellular Ca<sup>2+</sup> concentration in a dose-dependent manner, n = 4. (C) Analysis of intracellular Ca<sup>2+</sup> concentration showed that Jujuboside B increased intracellular Ca<sup>2+</sup> concentration in a dose-dependent manner, n = 4. (D) Representative confocal image showed that EGTA inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase. (E) Fluorescence intensity curves showed that EGTA significantly inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase, n = 4. (F) Analysis of intracellular Ca<sup>2+</sup> concentration showed that EGTA significantly inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase, ** <i>P</i> < 0.01, n = 4. (G) Representative confocal image showed that SKF96365 inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase. (H) Fluorescence intensity curves showed that SKF96365 significantly inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase, n = 4. (I) Analysis of intracellular Ca<sup>2+</sup> concentration showed that SKF96365 significantly inhibited Jujuboside B-induced intracellular Ca<sup>2+</sup> increase, ** <i>P</i> < 0.01, n = 4.</p> 2016-02-22 09:42:27 rat thoracic aorta rings L-NAME HAEC Griess reagent method rat thoracic aorta ring Activating Endothelial Nitric Oxide Synthase Jujuboside B nitric oxide synthase Jujuboside B DAMP Wire myograph system TRPC EGTA KN attenuated Jujuboside B-induced vasodilation SKF 96365. Jujuboside B-induced vasodilation tension laser confocal imaging microscopy