TY - DATA T1 - Secretome-Based Identification and Characterization of Potential Biomarkers in Thyroid Cancer PY - 2010/11/05 AU - Lawrence Kashat AU - Anthony K.-C. So AU - Olena Masui AU - X. Simon Wang AU - Jun Cao AU - Xianwang Meng AU - Christina MacMillan AU - Laurie E. Ailles AU - K. W. Michael Siu AU - Ranju Ralhan AU - Paul G. Walfish UR - https://acs.figshare.com/articles/journal_contribution/Secretome_Based_Identification_and_Characterization_of_Potential_Biomarkers_in_Thyroid_Cancer/2716588 DO - 10.1021/pr100529t.s005 L4 - https://ndownloader.figshare.com/files/4392538 KW - CAL 62 cells KW - cytoplasmic expression KW - TPC KW - thyroid carcinomas KW - warranting future analysis KW - PTMA KW - thyroid cancer patients KW - NOD KW - thyroid cancer cell lines KW - protein KW - Thyroid CancerIn search KW - thyroid cancer biomarkers N2 - In search of thyroid cancer biomarkers, proteins secreted by thyroid cancer cell lines, papillary-derived TPC-1 and anaplastic-derived CAL62, were analyzed using liquid chromatography−tandem mass spectrometry. Of 46 high-confidence identifications, 6 proteins were considered for verification in thyroid cancer patients’ tissue and blood. The localization of two proteins, nucleolin and prothymosin-α (PTMA), was confirmed in TPC-1 and CAL62 cells by confocal microscopy and immunohistochemically in xenografts of TPC-1 cells in NOD/SCID/γ mice and human thyroid cancers (48 tissues). Increased nuclear and cytoplasmic expression of PTMA was observed in anaplastic compared to papillary and poorly differentiated carcinomas. Nuclear expression of nucleolin was observed in all subtypes of thyroid carcinomas, along with faint cytoplasmic expression in anaplastic cancers. Importantly, PTMA, nucleolin, clusterin, cysteine-rich angiogenic inducer 61, enolase 1, and biotinidase were detected in thyroid cancer patients’ sera, warranting future analysis to confirm their potential as blood-based thyroid cancer markers. In conclusion, we demonstrated the potential of secretome analysis of thyroid cancer cell lines to identify novel proteins that can be independently verified in cell lines, xenografts, tumor tissues, and blood samples of thyroid cancer patients. These observations support their potential utility as minimally invasive biomarkers for thyroid carcinomas and their application in management of these diseases upon future validation. ER -