Surface Functionalization Using Catalyst-Free Azide−Alkyne Cycloaddition Alexander Kuzmin Andrei Poloukhtine Margreet A. Wolfert Vladimir V. Popik 10.1021/bc100306u.s001 https://acs.figshare.com/articles/journal_contribution/Surface_Functionalization_Using_Catalyst_Free_Azide_Alkyne_Cycloaddition/2712262 The utility of catalyst-free azide−alkyne [3 + 2] cycloaddition for the immobilization of a variety of molecules onto a solid surface and microbeads was demonstrated. In this process, the surfaces are derivatized with aza-dibenzocyclooctyne (ADIBO) for the immobilization of azide-tagged substrates via a copper-free click reaction. Alternatively, ADIBO-conjugated molecules are anchored to the azide-derivatized surface. Both immobilization techniques work well in aqueous solutions and show excellent kinetics under ambient conditions. We report an efficient synthesis of aza-dibenzocyclooctyne (ADIBO), thus far the most reactive cyclooctyne in cycloaddition to azides. We also describe convenient methods for the conjugation of ADIBO with a variety of molecules directly or via a PEG linker. 2010-11-17 00:00:00 surface cycloaddition reactive cyclooctyne azide variety immobilization techniques work ADIBO molecule ambient conditions Surface Functionalization PEG linker