10.1021/es1037595.s001
Maarten De Jonge
Maarten De
Jonge
Marleen Eyckmans
Marleen
Eyckmans
Ronny Blust
Ronny
Blust
Lieven Bervoets
Lieven
Bervoets
Are Accumulated Sulfide-Bound Metals Metabolically Available in the Benthic Oligochaete <i>Tubifex tubifex</i>?
American Chemical Society
2011
Ni
SEMMe
MSF
15 Flemish lowland rivers
worm tissue
benthic oligochaete Tubifex tubifex
subcellular
fraction
Ag
induction
BDM
cytosolic MTLP concentrations
Cr
Co
detoxified
Pb
Zn
accumulation
AVS
anoxic
sediment
Cu
2011-04-01 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Are_Accumulated_Sulfide_Bound_Metals_Metabolically_Available_in_the_Benthic_Oligochaete_i_Tubifex_tubifex_i_/2672158
The present study evaluates the relationship between metal-binding sediment characteristics like acid volatile sulfides (AVS), metal accumulation, and internal metal distribution in the benthic oligochaete <i>Tubifex tubifex</i> and relates this accumulation to the induction of metallothionein-like proteins (MTLPs). In total, 15 Flemish lowland rivers were sampled. Cd, Cu, Zn, Pb, Ni, As, Cr, Co, and Ag concentrations were measured in environmental fractions (water and sediment) and worm tissue (both total and subcellular fractions). Furthermore, total cytosolic MTLP concentrations were measured in the worm tissue. Our results showed that Cd, Pb, Ni, and Cr were mainly stored as biological detoxified metal (BDM) while Cu, Zn, As, and Ag were mostly available in the metal sensitive fraction (MSF). A remarkable difference in the subcellular distribution of accumulated Cd, Ni, and Co between anoxic (SEM<sub>Me</sub> − AVS < 0; mostly stored as BDM) and oxic (SEM<sub>Me</sub> − AVS > 0; mostly stored in the MSF) sediments was noticed. Moreover, a rapid increase in MTLP induction was found when SEM<sub>Tot</sub> − AVS > 0. Our results indicate that the accumulated sulfide-bound metals were detoxified and little available to the metabolism of <i>T. tubifex</i> under anoxic conditions.