%0 Journal Article %A Zhu, Lei %A Xie, Jin %A Swierczewska, Magdalena %A Zhang, Fan %A Lin, Xin %A Fang, Xuexun %A Niu, Gang %A Lee, Seulki %A Chen, Xiaoyuan %D 2011 %T Dual-Functional, Receptor-Targeted Fluorogenic Probe for In Vivo Imaging of Extracellular Protease Expressions %U https://acs.figshare.com/articles/journal_contribution/Dual_Functional_Receptor_Targeted_Fluorogenic_Probe_for_In_Vivo_Imaging_of_Extracellular_Protease_Expressions/2641801 %R 10.1021/bc200005w.s001 %2 https://ndownloader.figshare.com/files/4294036 %K MMP %K 87MG %K vivo fluorogenic probe %K Extracellular Protease ExpressionsHerein %K extracellular proteases %K receptor binding affinity %K α V β3 integrins %K tumor regions %K α V β3 integrin %X Herein, we report a new type of in vivo fluorogenic probe that enables simultaneous and active targeting of overexpressed receptors, αVβ3 integrins, and extracellular proteases, matrix metalloproteinases (MMPs), in the tumor regions. This c(RGDyK)-conjugated MMP fluorogenic probe efficiently targets the tumor regions with high retention time while maintaining receptor binding affinity and substrate activity. The probe minimizes nonspecific accumulation, thus demonstrating improved tumor-to-background signal ratio (T/N) in both αVβ3 integrin- and MMP-overexpressing U87MG tumor-bearing mouse model. This strategy can be easily tuned for a wide array of applications targeting various receptors and extracellular proteases in vivo. %I ACS Publications