Anti-DNA:RNA Antibodies and Silicon Photonic Microring Resonators: Increased Sensitivity for Multiplexed microRNA Detection Abraham J. Qavi Jared T. Kindt Martin A. Gleeson Ryan C. Bailey 10.1021/ac201340s.s001 https://acs.figshare.com/articles/journal_contribution/Anti_DNA_RNA_Antibodies_and_Silicon_Photonic_Microring_Resonators_Increased_Sensitivity_for_Multiplexed_microRNA_Detection/2627084 In this paper, we present a method for the sensitive detection of microRNAs (miRNAs) utilizing an antibody that specifically recognizes DNA:RNA heteroduplexes and a silicon photonic microring resonator array transduction platform. Microring resonator arrays are covalently functionalized with DNA capture probes that are complementary to solution phase miRNA targets. Following hybridization on the sensor, the anti-DNA:RNA antibody is introduced and binds selectively to the heteroduplexes, giving a larger signal than the original miRNA hybridization due to the increased mass of the antibody, as compared to the 22-mer oligoribonucleotide. Furthermore, the secondary recognition step is performed in neat buffer solution and at relatively higher antibody concentrations, facilitating the detection of miRNAs of interest. The intrinsic sensitivity of the microring resonator platform coupled with the amplification provided by the anti-DNA:RNA antibodies allows for the detection of microRNAs at concentrations as low as 10 pM (350 amol). The simplicity and sequence generality of this amplification method position it as a promising tool for high-throughput, multiplexed miRNA analysis as well as a range of other RNA based detection applications. 2011-08-01 00:00:00 miRNA hybridization multiplexed miRNA analysis silicon photonic microring resonator array transduction platform sequence generality covalently functionalized Silicon Photonic Microring Resonators solution phase miRNA targets heteroduplexe DNA antibody concentrations detection applications microring resonator platform Multiplexed microRNA DetectionIn amplification method position Microring resonator arrays buffer solution recognition step