10.1021/ac201340s.s001
Abraham J. Qavi
Abraham J.
Qavi
Jared T. Kindt
Jared T.
Kindt
Martin A. Gleeson
Martin A.
Gleeson
Ryan C. Bailey
Ryan C.
Bailey
Anti-DNA:RNA Antibodies and Silicon Photonic Microring Resonators: Increased Sensitivity for Multiplexed microRNA Detection
American Chemical Society
2011
miRNA hybridization
multiplexed miRNA analysis
silicon photonic microring resonator array transduction platform
sequence generality
covalently functionalized
Silicon Photonic Microring Resonators
solution phase miRNA targets
heteroduplexe
DNA
antibody concentrations
detection applications
microring resonator platform
Multiplexed microRNA DetectionIn
amplification method position
Microring resonator arrays
buffer solution
recognition step
2011-08-01 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Anti_DNA_RNA_Antibodies_and_Silicon_Photonic_Microring_Resonators_Increased_Sensitivity_for_Multiplexed_microRNA_Detection/2627084
In this paper, we present a method for the sensitive detection of microRNAs (miRNAs) utilizing an antibody that specifically recognizes DNA:RNA heteroduplexes and a silicon photonic microring resonator array transduction platform. Microring resonator arrays are covalently functionalized with DNA capture probes that are complementary to solution phase miRNA targets. Following hybridization on the sensor, the anti-DNA:RNA antibody is introduced and binds selectively to the heteroduplexes, giving a larger signal than the original miRNA hybridization due to the increased mass of the antibody, as compared to the 22-mer oligoribonucleotide. Furthermore, the secondary recognition step is performed in neat buffer solution and at relatively higher antibody concentrations, facilitating the detection of miRNAs of interest. The intrinsic sensitivity of the microring resonator platform coupled with the amplification provided by the anti-DNA:RNA antibodies allows for the detection of microRNAs at concentrations as low as 10 pM (350 amol). The simplicity and sequence generality of this amplification method position it as a promising tool for high-throughput, multiplexed miRNA analysis as well as a range of other RNA based detection applications.