10.1371/journal.pone.0042454.g001
Romina Nassini
Romina
Nassini
Pamela Pedretti
Pamela
Pedretti
Nadia Moretto
Nadia
Moretto
Camilla Fusi
Camilla
Fusi
Chiara Carnini
Chiara
Carnini
Fabrizio Facchinetti
Fabrizio
Facchinetti
Arturo Roberto Viscomi
Arturo
Roberto Viscomi
Anna Rita Pisano
Anna
Rita Pisano
Susan Stokesberry
Susan
Stokesberry
Charlott Brunmark
Charlott
Brunmark
Naila Svitacheva
Naila
Svitacheva
Lorcan McGarvey
Lorcan
McGarvey
Riccardo Patacchini
Riccardo
Patacchini
Anders B. Damholt
Anders
B. Damholt
Pierangelo Geppetti
Pierangelo
Geppetti
Serena Materazzi
Serena
Materazzi
TRPA1 channel expression in non-neuronal cells of the human and mouse respiratory tract.
Public Library of Science
2012
non-neuronal
cells
respiratory
2012-08-14 00:44:30
Figure
https://plos.figshare.com/articles/figure/_TRPA1_channel_expression_in_non_neuronal_cells_of_the_human_and_mouse_respiratory_tract_/262670
<p>(<b>A</b>) Total RNAs were extracted from primary small airways epithelial cells (SAEC), human type II alveolar epithelial cells (A549), human primary smooth muscle cells (HBSMC), human embryonic lung fibroblasts (IMR90) and primary normal human lung fibroblasts (NHLF) and relative TRPA1 mRNA amounts were measured by Taqman Real-Time PCR assay. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042454#s2" target="_blank">Results</a> are normalized to the reference gene, β-actin. Each column represents mean ± SEM of n>2 independent experiments. Immunohistochemical analysis of TRPA1 expression in samples taken from human (<b>B</b>) or <i>Trpa1<sup>+/+</sup></i> and <i>Trpa1<sup>−/−</sup></i> mouse airways and lung (<b>C</b>). Representative images of TRPA1 immunostaining show intense staining in epithelial and smooth muscle cells in human tissue. No staining is detected in human samples incubated with the normal serum peptide (Negative control). (<b>C</b>) Incubation with TRPA1 antibody shows a strong staining in epithelial and smooth muscle cells in tissues taken from <i>Trpa1<sup>+/+</sup></i> mice, but not in those from <i>Trpa1<sup>−/−</sup></i> mice. Preadsorption of the TRPA1 antibody with the peptide used for immunization abolished staining (Peptide). Staining for cytokeratin and α-smooth muscle actin (α-SMA) overlaps with the TRPA1 staining in the bronchial epithelium and smooth muscle layer in serial section of human and mice airways/lung tissues (<b>B</b> and <b>C</b>). Scale bar 100 µm.</p>