Tatewaki, Naoto Konishi, Tetsuya Nakajima, Yuki Nishida, Miyako Saito, Masafumi Eitsuka, Takahiro Sakamaki, Toshiyuki Ikekawa, Nobuo Nishida, Hiroshi Effect of squalene on G2/M checkpoint activity. <p>G2/M checkpoint analysis was performed by flow cytometry. (A) The percentage of mitotic cells was estimated according to the phosphorylation of histone H3 in Ser10-positive cells. A549 adenocarcinoma cells were counterstained with PI for visualization of DNA content. The cells were treated with 100 μM squalene for 4 h before and 1 h after γIR (0 and 6 Gy). (B) Data are expressed as the percentage of mitotic cells in the total number of cells. Data are presented as the mean ± S.D. of three independent experiments (*P < 0.05 vs. untreated control).</p> p 53;anticancer therapies;ATM effectors;protein phosphatase;Squalene;Chk 1;kinase activity;Wip 1 expression;squalene;ATM activation;Wip 1 Phosphatase Ataxia telangiectasia;checkpoint inhibitors;DNA damage;intracellular induction;549 cells;DNA damage response;cell viability;master controller;cell treatment;IR;cell cycle;complement anticancer therapies 2016-01-29
    https://plos.figshare.com/articles/figure/Effect_of_squalene_on_G2_M_checkpoint_activity_/2624940
10.1371/journal.pone.0147570.g004