Qi, Jianjun Han, Myung-Shin Chang, Yu-Cheng Tung, Ching-Hsuan Developing Visible Fluorogenic ‘Click-On’ Dyes for Cellular Imaging New fluorogenic dyes were designed and synthesized based on Cu(I)-catalyzed ‘click’ reaction. Conjugating weakly fluorescent benzothiazole derivatives with an electron-deficient alkyne group at the 2-position with azide-containing molecules in aqueous solution form ‘click-on’ fluorescent adducts. Model reactions and cell culture experiment indicated that the developed ‘click-on’ dye could be applied to labeling various biomolecules, such as nucleic acids, proteins, and other molecules, in cells. cell culture experiment;adduct;biomolecule;protein;acid;Model reactions;Fluorogenic;benzothiazole derivatives;Conjugating;molecule;Cellular ImagingNew fluorogenic dyes;Dye;solution;alkyne;Visible;Cu 2011-09-21
    https://acs.figshare.com/articles/journal_contribution/Developing_Visible_Fluorogenic_Click_On_Dyes_for_Cellular_Imaging/2612014
10.1021/bc200282t.s001