%0 Journal Article %A Qi, Jianjun %A Han, Myung-Shin %A Chang, Yu-Cheng %A Tung, Ching-Hsuan %D 2011 %T Developing Visible Fluorogenic ‘Click-On’ Dyes for Cellular Imaging %U https://acs.figshare.com/articles/journal_contribution/Developing_Visible_Fluorogenic_Click_On_Dyes_for_Cellular_Imaging/2612014 %R 10.1021/bc200282t.s001 %2 https://ndownloader.figshare.com/files/4261909 %K cell culture experiment %K adduct %K biomolecule %K protein %K acid %K Model reactions %K Fluorogenic %K benzothiazole derivatives %K Conjugating %K molecule %K Cellular ImagingNew fluorogenic dyes %K Dye %K solution %K alkyne %K Visible %K Cu %X New fluorogenic dyes were designed and synthesized based on Cu(I)-catalyzed ‘click’ reaction. Conjugating weakly fluorescent benzothiazole derivatives with an electron-deficient alkyne group at the 2-position with azide-containing molecules in aqueous solution form ‘click-on’ fluorescent adducts. Model reactions and cell culture experiment indicated that the developed ‘click-on’ dye could be applied to labeling various biomolecules, such as nucleic acids, proteins, and other molecules, in cells. %I ACS Publications