Detection of DNA Targets Hybridized to Solid Surfaces Using Optical Images of Liquid Crystals
Siok Lian Lai
Wei Ling Tan
Kun-Lin Yang
10.1021/am200571h.s001
https://acs.figshare.com/articles/journal_contribution/Detection_of_DNA_Targets_Hybridized_to_Solid_Surfaces_Using_Optical_Images_of_Liquid_Crystals/2609887
In this paper, we report a method of detecting DNA targets hybridized to a solid surface by using liquid crystals (LC). The detection principle is based on different interference colors of LC supported on surfaces decorated with single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA). However, the contrast between the ssDNA and dsDNA is not obvious, unless DNA-streptavidin complexes are introduced to the dsDNA to increase the surface mass density. Two different approaches of introducing streptavidin to the system are studied and compared. We find that by premixing the biotin-labeled DNA targets with streptavidin prior to the DNA hybridization, branched-streptavidin complexes are formed and clear LC signal can be observed. This LC-based DNA detection principle represents an important step toward the development of a simple, instrument- and fluorophore-free DNA detection method.
2011-09-28 00:00:00
DNA hybridization
Optical Images
Solid Surfaces
interference colors
dsDNA
Liquid CrystalsIn
detection principle
DNA targets hybridized
LC signal
surface mass density