DIDS does not effect intracellular expression of pro- or active MMP-2 or -9 isoforms. Matthew E. Pamenter Julie Ryu Serena T. Hua Guy A. Perkins Vincent L. Mendiola Xiang Q. Gu Mark H. Ellisman Gabriel G. Haddad 10.1371/journal.pone.0043995.g004 https://plos.figshare.com/articles/figure/_DIDS_does_not_effect_intracellular_expression_of_pro_or_active_MMP_2_or_9_isoforms_/258047 <p>IS and STS treatment increased intracellular expression of proMMP-2 and -9 isoforms, as well as the active form of MMP-2. DIDS did not effect these changes. (<b>A</b>) Sample Western blots of MMP-2 and -9 protein expression. Latent and active isoforms of MMPs were detected at 66 and 62 kDA (MMP-2) and 96 and 88 kDA (MMP-9), respectively. (<b>B&C</b>) Summaries of neuronal latent and active MMP-2 (B) and MMP-9 (C) protein isoform expression from analysis of cellular fractions normalized to the cellular expression of α-actin on the same blot. Data are presented as fold-change relative to untreated controls. Data are mean ±SEM from 3-5 separate 6-hr experiments. Asterisks (*) indicate significant difference from normoxic controls (<i>p</i><0.05). Treatments as per Fig. 1 caption.</p> 2012-08-24 02:14:07 intracellular pro- mmp-2 -9