All Optical Determination of Microscopic and Macroscopic Structure of Chiral, Polar Microcrystals from Achiral, Nonpolar Molecules Stijn Van Cleuvenbergen Gunther Hennrich Pieter Willot Guy Koeckelberghs Koen Clays Thierry Verbiest Monique A. van der Veen 10.1021/jp302665t.s001 https://acs.figshare.com/articles/journal_contribution/All_Optical_Determination_of_Microscopic_and_Macroscopic_Structure_of_Chiral_Polar_Microcrystals_from_Achiral_Nonpolar_Molecules/2515756 Organic microcrystals obtained from an octupolar molecule are studied by polarized nonlinear optical microscopy. While two-photon fluorescence microscopy is employed to verify the monocrystallinity of the analyzed domains, second-harmonic generation microscopy allowed determination of the point group symmetry of the crystallites. This combined analysis revealed that the achiral, octupolar molecules form chiral and polar conglomerate structures; the former are confirmed by circular dichroism spectroscopy. We additionally present a convenient and straightforward image analysis protocol, based on polarization dependent second-harmonic generation microscopy. This allows assessing the local organization and homogeneity of crystalline structures, which is highly relevant for technological applications, with high resolution and sensitivity. 2012-06-07 00:00:00 generation Polar Microcrystals dichroism spectroscopy octupolar molecule Macroscopic Structure image analysis protocol conglomerate structures octupolar molecules form chiral Optical Determination microscopy Nonpolar MoleculesOrganic microcrystals point group symmetry