10.1021/ac300608q.s003 Oscar Filevich Oscar Filevich Guillermo Carrone Guillermo Carrone Victoria Andino Pavlovsky Victoria Andino Pavlovsky Roberto Etchenique Roberto Etchenique Fast Optical pH Manipulation and Imaging American Chemical Society 2016 pH images 5 pH units 1200 fps LED illumination system nanosecond time scale imaging system FIA pH manipulation Fast Optical pH Manipulation Flow Injection Analysis photoactive Ruthenium 2016-02-20 18:02:56 Media https://acs.figshare.com/articles/media/Fast_Optical_pH_Manipulation_and_Imaging/2508667 We describe a complete system for optical pH manipulation and imaging. The system consists of a photoactive Ruthenium complex capable of inducing a change of more than 5 pH units at the nanosecond time scale. A compatible imaging system acquires microscopic pH images at 1200 fps using a nonexpensive commercial digital camera and an LED illumination system. We use the system as a superb tool to investigate flow in Flow Injection Analysis (FIA) models.