TY - DATA T1 - Endocytic Pathway and Resistance to Cholesterol Depletion of Cholesterol Derived Cationic Lipids for Gene Delivery PY - 2012/12/03 AU - Yun-Ui Bae AU - Bieong-Kil Kim AU - Jong-Won Park AU - Young-Bae Seu AU - Kyung-Oh Doh UR - https://acs.figshare.com/articles/journal_contribution/Endocytic_Pathway_and_Resistance_to_Cholesterol_Depletion_of_Cholesterol_Derived_Cationic_Lipids_for_Gene_Delivery/2464726 DO - 10.1021/mp300458h.s001 L4 - https://ndownloader.figshare.com/files/4107418 KW - cholesterol depletion KW - resistance KW - cationic KW - pathway KW - transfection KW - lipid KW - Cholesterol Derived Cationic Lipids KW - DOTAP N2 - Cholesterol-based cationic lipids have been widely used because of biocompatibility and serum resistance. However, the reason for the effectiveness of cholesterol-based cationic lipids remains unclear. We compared the transfection route of CHOL-E, a cholesterol-based cationic lipid having an amine head and an ether linker, with that of DOTAP. The luciferase assay with chemical inhibitors and microscopic observation of pathway markers revealed that clathrin mediated endocytosis is the main pathway for CHOL-E and DOTAP. However, CHOL-E showed resistance to cholesterol depletion by methyl-β-cyclodextrin. Furthermore, CHOL-E recovered the transfection efficiency of DOTAP from cholesterol depletion. These results suggested that superior transfection of CHOL-E might be partly derived from effects on the cell membrane. ER -