10.1371/journal.pone.0043860.g002 Aarti Gautam Aarti Gautam Saurabh Dixit Saurabh Dixit Monica Embers Monica Embers Rajeev Gautam Rajeev Gautam Mario T. Philipp Mario T. Philipp Shree R. Singh Shree R. Singh Lisa Morici Lisa Morici Vida A. Dennis Vida A. Dennis IL-10 is more highly expressed by macrophages of the Lyme disease-resistant mouse than by those of the disease-susceptible mouse. Public Library of Science 2013 macrophages lyme disease-resistant disease-susceptible 2013-02-19 23:16:11 Figure https://plos.figshare.com/articles/figure/_IL_10_is_more_highly_expressed_by_macrophages_of_the_Lyme_disease_resistant_mouse_than_by_those_of_the_disease_susceptible_mouse_/246345 <p>BMDM cultures were established as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043860#pone-0043860-g001" target="_blank">Fig. 1</a> and cell-free culture supernatants and RNA samples were collected after 24 h of incubation. (A) IL-10 protein concentrations were quantified using ELISA. (B) IL-10 mRNA gene transcripts were quantified by SABiosciences RT<sup>2</sup> Profiler™ PCR Array as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043860#pone-0043860-g001" target="_blank">Fig. 1B</a>. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043860#s2" target="_blank">Results</a> are presented as fold increase over control (the level in unstimulated cells). Data were analyzed using a one- or two-way ANOVA followed by a Bonferroni post-hoc test (GraphPad Prism 5). Significance was established at <i>P</i><0.001 = ***, <i>P</i><0.01 = ** and <i>P</i><0.05 = *. Each bar represents the mean ± standard deviation for duplicate cultures. Data are representative of two independent experiments, with similar results.</p>