Through Bond Energy Transfer:
A Convenient and Universal
Strategy toward Efficient Ratiometric Fluorescent Probe for Bioimaging
Applications
Yi-Jun Gong
Xiao-Bing Zhang
Cui-Cui Zhang
Ai-Li Luo
Ting Fu
Weihong Tan
Guo-Li Shen
Ru-Qin Yu
10.1021/ac302762d.s001
https://acs.figshare.com/articles/journal_contribution/Through_Bond_Energy_Transfer_A_Convenient_and_Universal_Strategy_toward_Efficient_Ratiometric_Fluorescent_Probe_for_Bioimaging_Applications/2459521
Fluorescence resonance energy transfer (FRET) strategy
has been
widely applied in designing ratiometric probes for bioimaging applications.
Unfortunately, for FRET systems, sufficiently large spectral overlap
is necessary between the donor emission and the acceptor absorption,
which would limit the resolution of double-channel images. The through-bond
energy transfer (TBET) system does not need spectral overlap between
donor and acceptor and could afford large wavelength difference between
the two emissions with improved imaging resolution and higher energy
transfer efficiency than that of the classical FRET system. It seems
to be more favorable for designing ratiometric probes for bioimaging
applications. In this paper, we have designed and synthesized a coumarin–rhodamine
(CR) TBET system and demonstrated that TBET is a convenient strategy
to design an efficient ratiometric fluorescent bioimaging probe for
metal ions. Such TBET strategy is also universal, since no spectral
overlap between the donor and the acceptor is necessary, and many
more dye pairs than that of FRET could be chosen for probe design.
As a proof-of-concept, Hg<sup>2+</sup> was chosen as a model metal
ion. By combining TBET strategy with dual-switch design, the proposed
sensing platform shows two well-separated emission peaks with a wavelength
difference of 110 nm, high energy transfer efficiency, and a large
signal-to-background ratio, which affords a high sensitivity for the
probe with a detection limit of 7 nM for Hg<sup>2+</sup>. Moreover,
by employing an Hg<sup>2+</sup>-promoted desulfurization reaction
as recognition unit, the probe also shows a high selectivity to Hg<sup>2+</sup>. All these unique features make it particularly favorable
for ratiometric Hg<sup>2+</sup> sensing and bioimaging applications.
It has been preliminarily used for a ratiometric image of Hg<sup>2+</sup> in living cells and practical detection of Hg<sup>2+</sup> in river
water samples with satisfying results.
2012-12-18 00:00:00
river water samples
overlap
wavelength difference
Hg
model metal ion
acceptor
energy transfer efficiency
Bond Energy Transfer
emission
bioimaging applications
Such TBET strategy
CR
FRET
Bioimaging ApplicationsFluorescence resonance energy transfer
donor
ratiometric probes