10.1021/jf304444s.s001
Nigel I. Joyce
Nigel I.
Joyce
Colin C. Eady
Colin C.
Eady
Patrick Silcock
Patrick
Silcock
Nigel
B. Perry
Nigel
B.
Perry
John W. van Klink
John
W. van Klink
Fast Phenotyping of LFS-Silenced
(Tearless) Onions
by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS)
American Chemical Society
2013
lachrymatory factor synthase
sulfenic acid condensates
proton transfer reaction
desorption electrospray ionization
PTR
MS
tearless onions
sampling
LFS
Allium
concentration
LF
variety
Desorption Electrospray Ionization Mass Spectrometry
dipropyl disulfide 12
DESI
2013-02-20 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Fast_Phenotyping_of_LFS_Silenced_Tearless_Onions_by_Desorption_Electrospray_Ionization_Mass_Spectrometry_DESI_MS_/2441647
Fast MS techniques have been applied to the analysis
of sulfur
volatiles in Allium species and varieties
to distinguish phenotypes. Headspace sampling by proton transfer reaction
(PTR) MS and surface sampling by desorption electrospray ionization
(DESI) MS were used to distinguish lachrymatory factor synthase (LFS)-silenced
(tearless; LFS−) onions from normal, LFS-active (tear-inducing;
LFS+), onions. PTR-MS showed lower concentrations of the lachrymatory
factor (LF, <b>3</b>) and dipropyl disulfide <b>12</b> from tearless onions. DESI-MS of the tearless onions confirmed the
decreased LF <b>3</b> and revealed much higher concentrations
of the sulfenic acid condensates. Using DESI-MS with MS<sup>2</sup> could distinguish zwiebelane ions from thiosulfinate ions. DESI-MS
gave reliable fast phenotyping of LFS+ versus LFS– onions by
simply scratching leaves and recording the extractable ions for <0.5
min. DESI-MS leaf compound profiles also allowed the rapid distinction
of a variety of <i>Allium</i> cultivars to aid plant breeding
selections.