Characterization of Genes for a Putative Hydroxycinnamoyl-coenzyme A Quinate Transferase and <i>p</i>‑Coumarate 3′-Hydroxylase and Chlorogenic Acid Accumulation in Tartary Buckwheat Yeon Bok Kim Aye Aye Thwe Ye Ji Kim Xiaohua Li Haeng Hoon Kim Phun Bum Park Tastsuro Suzuki Sun-Ju Kim Sang Un Park 10.1021/jf4000659.s001 https://acs.figshare.com/articles/journal_contribution/Characterization_of_Genes_for_a_Putative_Hydroxycinnamoyl_coenzyme_A_Quinate_Transferase_and_i_p_i_Coumarate_3_Hydroxylase_and_Chlorogenic_Acid_Accumulation_in_Tartary_Buckwheat/2420233 Tartary buckwheat (Fagopyrum tataricum Gaertn.) contains a high level of flavonoid compounds, which have beneficial and pharmacological effects on health. In this study, we isolated full-length cDNAs encoding hydroxycinnamoyl-coenzyme A quinate hydroxycinnamoyltransferase (HQT) and <i>p</i>-coumarate 3′-hydroxylase (C3H), which are involved in chlorogenic acid (CGA) biosynthesis. We examined the expression levels of HQT and C3H using real-time RT-PCR in different organs and sprouts of two tartary buckwheat cultivars (Hokkai T8 and T10) and analyzed CGA content using high-performance liquid chromatography. Among the organs, the flowers in both cultivars showed the highest levels of CGA. We concluded that the expression pattern of <i>FtHQT</i> and <i>FtC3H</i> did not match the accumulation pattern of CGA in different organs of T8 and T10 cultivars. Gene expression and CGA content varied between the cultivars. We presume that <i>FtHQT</i> and <i>FtC3H</i> levels might be controlled by multiple metabolic pathways in different organs of tartary buckwheat. Probably, <i>FtC3H</i> might have a greater effect on CGA biosynthesis than <i>FtHQT</i>. Our results will be helpful for a greater understanding of CGA biosynthesis in tartary buckwheat. 2013-05-01 00:00:00 tartary buckwheat cultivars FtC 3H CGA biosynthesis T 10 cultivars C 3H FtHQT expression tartary buckwheat CGA content HQT Hokkai T 8 FtC 3H levels Tartary BuckwheatTartary buckwheat