Characterization of Genes for a Putative Hydroxycinnamoyl-coenzyme
A Quinate Transferase and <i>p</i>‑Coumarate 3′-Hydroxylase
and Chlorogenic Acid Accumulation in Tartary Buckwheat
Yeon Bok Kim
Aye Aye Thwe
Ye Ji Kim
Xiaohua Li
Haeng Hoon Kim
Phun Bum Park
Tastsuro Suzuki
Sun-Ju Kim
Sang Un Park
10.1021/jf4000659.s001
https://acs.figshare.com/articles/journal_contribution/Characterization_of_Genes_for_a_Putative_Hydroxycinnamoyl_coenzyme_A_Quinate_Transferase_and_i_p_i_Coumarate_3_Hydroxylase_and_Chlorogenic_Acid_Accumulation_in_Tartary_Buckwheat/2420233
Tartary buckwheat (Fagopyrum tataricum Gaertn.) contains a high level
of flavonoid compounds, which have
beneficial and pharmacological effects on health. In this study, we
isolated full-length cDNAs encoding hydroxycinnamoyl-coenzyme A quinate
hydroxycinnamoyltransferase (HQT) and <i>p</i>-coumarate
3′-hydroxylase (C3H), which are involved in chlorogenic acid
(CGA) biosynthesis. We examined the expression levels of HQT and C3H
using real-time RT-PCR in different organs and sprouts of two tartary
buckwheat cultivars (Hokkai T8 and T10) and analyzed CGA content using
high-performance liquid chromatography. Among the organs, the flowers
in both cultivars showed the highest levels of CGA. We concluded that
the expression pattern of <i>FtHQT</i> and <i>FtC3H</i> did not match the accumulation pattern of CGA in different organs
of T8 and T10 cultivars. Gene expression and CGA content varied between
the cultivars. We presume that <i>FtHQT</i> and <i>FtC3H</i> levels might be controlled by multiple metabolic pathways
in different organs of tartary buckwheat. Probably, <i>FtC3H</i> might have a greater effect on CGA biosynthesis than <i>FtHQT</i>. Our results will be helpful for a greater understanding of CGA
biosynthesis in tartary buckwheat.
2013-05-01 00:00:00
tartary buckwheat cultivars
FtC 3H
CGA biosynthesis
T 10 cultivars
C 3H
FtHQT
expression
tartary buckwheat
CGA content
HQT
Hokkai T 8
FtC 3H levels
Tartary BuckwheatTartary buckwheat