10.1021/ja403585e.s001
Hongzhou Gu
Hongzhou
Gu
Kazuhiro Furukawa
Kazuhiro
Furukawa
Zasha Weinberg
Zasha
Weinberg
Daniel
F. Berenson
Daniel
F.
Berenson
Ronald R. Breaker
Ronald R.
Breaker
Small, Highly Active DNAs That Hydrolyze DNA
American Chemical Society
2016
pH
genomic instability
Active DNAs
hydrolytic degradation
consensus sequence
Hydrolyze DNADNA phosphoester bonds
hydrolyze DNA
Zn
deoxyribozyme
DNA regions
hydrolysis
2016-02-19 05:44:40
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Small_Highly_Active_DNAs_That_Hydrolyze_DNA/2404081
DNA phosphoester bonds are exceedingly
resistant to hydrolysis in the absence of chemical or enzymatic catalysts.
This property is particularly important for organisms with large genomes,
as resistance to hydrolytic degradation permits the long-term storage
of genetic information. Here we report the creation and analysis of
two classes of engineered deoxyribozymes that selectively and rapidly
hydrolyze DNA. Members of class I deoxyribozymes carry a catalytic
core composed of only 15 conserved nucleotides and attain an observed
rate constant (<i>k</i><sub>obs</sub>) of ∼1 min<sup>–1</sup> when incubated near neutral pH in the presence of
Zn<sup>2+</sup>. Natural DNA sequences conforming to the class I consensus
sequence and structure were found that undergo hydrolysis under selection
conditions (2 mM Zn<sup>2+</sup>, pH 7), which demonstrates that the
inherent structure of certain DNA regions might promote catalytic
reactions, leading to genomic instability.