%0 Online Multimedia %A Hernández-Ainsa, Silvia %A A. W. Bell, Nicholas %A Thacker, Vivek V. %A Göpfrich, Kerstin %A Misiunas, Karolis %A Fuentes-Perez, Maria Eugenia %A Moreno-Herrero, Fernando %A Keyser, Ulrich F. %D 2013 %T DNA Origami Nanopores for Controlling DNA Translocation %U https://acs.figshare.com/articles/media/DNA_Origami_Nanopores_for_Controlling_DNA_Translocation/2393944 %R 10.1021/nn401759r.s002 %2 https://ndownloader.figshare.com/files/4033639 %K DNA Origami Nanopores %K reversibly form %K binding sites %K Controlling DNA TranslocationWe %K pore size %K DNA origami nanopores %K DNA origami %K glass nanocapillaries %K DNA origami structures %K DNA origami nanopore %K imaging fluorescently %X We combine DNA origami structures with glass nanocapillaries to reversibly form hybrid DNA origami nanopores. Trapping of the DNA origami onto the nanocapillary is proven by imaging fluorescently labeled DNA origami structures and simultaneous ionic current measurements of the trapping events. We then show two applications highlighting the versatility of these DNA origami nanopores. First, by tuning the pore size we can control the folding of dsDNA molecules (“physical control”). Second, we show that the specific introduction of binding sites in the DNA origami nanopore allows selective detection of ssDNA as a function of the DNA sequence (“chemical control”). %I ACS Publications