Direct Delivery of Functional Proteins and Enzymes to the Cytosol Using Nanoparticle-Stabilized Nanocapsules
Rui Tang
Chang Soo Kim
David J. Solfiell
Subinoy Rana
Rubul Mout
Elih M. Velázquez-Delgado
Apiwat Chompoosor
Youngdo Jeong
Bo Yan
Zheng-Jiang Zhu
Chaekyu Kim
Jeanne A. Hardy
Vincent M. Rotello
10.1021/nn402753y.s001
https://acs.figshare.com/articles/journal_contribution/Direct_Delivery_of_Functional_Proteins_and_Enzymes_to_the_Cytosol_Using_Nanoparticle_Stabilized_Nanocapsules/2383636
Intracellular protein delivery is an important tool for both therapeutic and fundamental applications. Effective protein delivery faces two major challenges: efficient cellular uptake and avoiding endosomal sequestration. We report here a general strategy for direct delivery of functional proteins to the cytosol using nanoparticle-stabilized capsules (NPSCs). These NPSCs are formed and stabilized through supramolecular interactions between the nanoparticle, the protein cargo, and the fatty acid capsule interior. The NPSCs are ∼130 nm in diameter and feature low toxicity and excellent stability in serum. The effectiveness of these NPSCs as therapeutic protein carriers was demonstrated through the delivery of fully functional caspase-3 to HeLa cells with concomitant apoptosis. Analogous delivery of green fluorescent protein (GFP) confirmed cytosolic delivery as well as intracellular targeting of the delivered protein, demonstrating the utility of the system for both therapeutic and imaging applications.
2013-08-27 00:00:00
HeLa cells
cytosolic delivery
acid capsule
protein carriers
Functional Proteins
Analogous delivery
Direct Delivery
supramolecular interactions
Effective protein delivery
protein cargo
NPSC
endosomal sequestration
imaging applications
GFP