10.1021/nn402753y.s003
Rui Tang
Rui
Tang
Chang Soo Kim
Chang Soo
Kim
David J. Solfiell
David J.
Solfiell
Subinoy Rana
Subinoy
Rana
Rubul Mout
Rubul
Mout
Elih M. Velázquez-Delgado
Elih M.
Velázquez-Delgado
Apiwat Chompoosor
Apiwat
Chompoosor
Youngdo Jeong
Youngdo
Jeong
Bo Yan
Bo
Yan
Zheng-Jiang Zhu
Zheng-Jiang
Zhu
Chaekyu Kim
Chaekyu
Kim
Jeanne A. Hardy
Jeanne A.
Hardy
Vincent M. Rotello
Vincent M.
Rotello
Direct Delivery of Functional Proteins and Enzymes to the Cytosol Using Nanoparticle-Stabilized Nanocapsules
American Chemical Society
2013
HeLa cells
cytosolic delivery
acid capsule
protein carriers
Functional Proteins
Analogous delivery
Direct Delivery
supramolecular interactions
Effective protein delivery
protein cargo
NPSC
endosomal sequestration
imaging applications
GFP
2013-08-27 00:00:00
Media
https://acs.figshare.com/articles/media/Direct_Delivery_of_Functional_Proteins_and_Enzymes_to_the_Cytosol_Using_Nanoparticle_Stabilized_Nanocapsules/2383630
Intracellular protein delivery is an important tool for both therapeutic and fundamental applications. Effective protein delivery faces two major challenges: efficient cellular uptake and avoiding endosomal sequestration. We report here a general strategy for direct delivery of functional proteins to the cytosol using nanoparticle-stabilized capsules (NPSCs). These NPSCs are formed and stabilized through supramolecular interactions between the nanoparticle, the protein cargo, and the fatty acid capsule interior. The NPSCs are ∼130 nm in diameter and feature low toxicity and excellent stability in serum. The effectiveness of these NPSCs as therapeutic protein carriers was demonstrated through the delivery of fully functional caspase-3 to HeLa cells with concomitant apoptosis. Analogous delivery of green fluorescent protein (GFP) confirmed cytosolic delivery as well as intracellular targeting of the delivered protein, demonstrating the utility of the system for both therapeutic and imaging applications.