10.1371/journal.pone.0047320.g004 Lori L. Tortorella Lori L. Tortorella Nina H. Pipalia Nina H. Pipalia Sushmita Mukherjee Sushmita Mukherjee Ira Pastan Ira Pastan David Fitzgerald David Fitzgerald Frederick R. Maxfield Frederick R. Maxfield Comparison of cell toxicity at comparable levels of immunotoxin binding. Public Library of Science 2013 toxicity levels immunotoxin 2013-02-19 22:02:50 Figure https://plos.figshare.com/articles/figure/_Comparison_of_cell_toxicity_at_comparable_levels_of_immunotoxin_binding_/232792 <p>Tac-TGN38 and Tac-furin cells were treated with the indicated dose of LMB-2. After a 24 hour incubation with immunotoxin, cells were washed, and the cells remaining in the well were used for <b>A</b>) Hoechst-based cell count assay or <b>B</b>) LDH assay as described in Methods. Panels A and B show the relative cytotoxicity plotted against the amount of LMB-2 adjusted to reflect the different Tac expression levels (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047320#pone-0047320-g003" target="_blank">Figure 3C</a>) in the cell lines ([LMB-2] × Tac expression). Here the cytotoxicity has been normalized so that a value of 1 represents the maximum killing observed with the highest dose of LMB-2 for each cell line (relative cytotoxicity). The data points represent the cytotoxicity values averaged over several experiments, cell count assay (n = 9), LDH assay (n = 8). The standard error of the mean is shown.</p>