%0 Figure %A I. Nita, Iulia %A Hershfinkel, Michal %A Fishman, Daniel %A Ozeri, Eyal %A Rutter, Guy A. %A L. Sensi, Stefano %A Khananshvili, Daniel %A C. Lewis, Eli %A Sekler, Israel %D 2013 %T Effect of NCLX expression or activity on glucose dependent cytosolic calcium responses. %U https://plos.figshare.com/articles/figure/_Effect_of_NCLX_expression_or_activity_on_glucose_dependent_cytosolic_calcium_responses_/232788 %R 10.1371/journal.pone.0046649.g004 %2 https://ndownloader.figshare.com/files/562304 %K nclx %K glucose %K cytosolic %K calcium %X

A. Real time PCR analysis of mRNA NCLX expression normalized to GAPDH in pancreatic primary β cells transfected with siNCLX vs. siControl, n = 3 (*P<0.05). B. Silencing NCLX expression inhibits glucose-induced Ca2+ entry in primary β cells. Representative fluorescent traces of cytosolic Ca2+ in pancreatic primary β cells transfected with either siNCLX or siControl loaded with Fura 2 AM and stimulated with high glucose following the same experimental paradigm described in Fig. 2A. Insert. Shows representative images of MIN6 cells co-transfected with the Dharmacon siGLO Red transfection reagent. The scale bar is 10 µm. C. NCLX dominant negative construct inhibits glucose dependent cytosolic Ca2+ changes in primary β cells. Representative fluorescent traces of primary β cells transfected with dnNCLX or control vector (pcDNA) loaded with Fura 2 AM and treated with high glucose when indicated. D. Averaged rates of cytosolic Ca2+ responses of Fig. 4B, n = 10 (*P<0.05). E. Averaged rates of cytosolic Ca2+ responses of Fig. 4C, n = 10 (*P<0.05). F. Averaged cytosolic Ca2+ amplitudes of Fig. 4B, n = 10 (*P<0.05). G. Averaged cytosolic Ca2+ amplitudes of Fig. 4C, n = 10 (*P<0.05).

%I PLOS ONE